Legislation that goes into effect on Thursday bars UNRWA from operating in the country. That will affect aid delivery to Gaza and beyond.
The law that bans the United Nations agency that aids Palestinian refugees in Gaza went into effect on Wednesday.
The UN has said that its aid agency for Palestinian refugees, UNRWA, will continue working in all Palestinian territories, including east Jerusalem, as Israel cut ties with the organisation.
In the Shuafat refugee camp, a hardscrabble district in east Jerusalem surrounded by a hulking concrete wall, intense security checks make venturing out exasperating.
Laws passed in October went into effect, barring the U.N. agency from the Jewish state and barring state officials from communicating with the group.
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Israel has banned a key UN agency from operating in Israel, making the future of its operations in Gaza and the West Bank unclear.
The agency, UNRWA, will be banned from operating on Israeli soil, and contact between it and Israeli officials will also be forbidden.
The Israeli government ban on UNRWA over national security concerns and links to terrorism began Jan. 30. UNRWA has called the move by Jerusalem as "disastrous."
Tens of thousands of Palestinian refugees in Israeli-occupied East Jerusalem were set to lose education, healthcare and other services provided by U.N. agency UNRWA as an Israeli ban on the organisation takes effect on Thursday.
UN refugee agency says it has not received any official statement on how Israel's parliament will implement ban