The director Charles Burnett’s deeply humane, singular film from 1999, starring James Earl Jones, is finally receiving a theatrical release. By Manohla Dargis Miles Teller and Anya Taylor-Joy ...
For immunostaining, brain slices were permeabilized and blocked by incubating them in a blocking buffer (5% normal donkey serum, 0.3% Triton-X in PBS) for 2 h at room temperature on a shaker. Blocking ...
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